Chromatography and Its Applications [biological] by S. Dhanarasu

By S. Dhanarasu

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Phytochemistry, Vol. 25, pp. 133-135. P. (2000). Medicinal Plants and Phytomedicines. Linking Plant Biochemistry and Physiology to Human Health. Plant Physiology, Vol. 124, pp. 507-514. Y. K. (2003). Terpenoids from the Seeds of Artemisia annua. Phytochemistry, Vol. 64, pp. 303-323. D. M. (2003). Identification and Quantification of Carotenoids, Tocopherols and Chlorophylls in Commonly Consumed Fruits and Vegetables. Phytochemistry, Vol. 62, pp. 939-947. F. & Lacroix, M. (2011). Effect of Juice Processing on the Cancer Chemopreventive Effect of Cranberry.

The dried and powdered leaves of Crategus davisii were extracted with petroleum ether and then with EtOH. The petroleum ether extract was concentrated and extracted with 60% EtOH. The aqueous extract was concentrated and extracted with CHCl3 (Extract A). The EtOH extract was concentrated and extracted with toluene, CHCl3 (Extract B) and EtOAc (Extract C) successively. Extract A was chromatographed first by vacuum liquid chromatography (VLC) on silica gel with Petroleum ether-CHCl3-MeOH mixtures.

2002). Methanolic extract of the rhizomes of C. racemosa was concentrated under vacuum and the viscous concentrate was passed through a Diaion HP-20 column and eluted with 30% MeOH, 50% MeOH, MeOH, EtOH and EtOAc successively. Fractions eluted with 50% MeOH were subjected to column chromatography on silica gel eluting with stepwise gradient mixtures of CHCl3-MeOH- H2O (19:1:0, 9:1:0, 40:10:1, 20:10:1) and finally with MeOH to afford 7 fractions. Fraction 4 rechromatographed on silica gel column chromatography eluting with CHCl3-MeOH (30:1, 10:1, 5:1) and ODS silica gel with MeOHH2O (8:5) to give 25-O-acetyl-12β-hydroxycimigenol 3-O-α-L-arabinopyranoside.

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