By Maria Teresa Giardi, Elena Piletska
Biotechnological purposes of Photosynthetic Proteins: Biochips, Biosensors and Biodevices presents an outline of the new photosystem II study and the platforms on hand for the bioassay of toxins utilizing biosensors which are in accordance with the photochemical job. the knowledge provided during this e-book serves as a foundation for the advance of a advertisement biosensor to be used in quick pre-screening analyses of photosystem II toxins, minimising expensive and time-consuming laboratory analyses.
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Biotechnological functions of Photosynthetic Proteins: Biochips, Biosensors and Biodevices presents an outline of the hot photosystem II learn and the structures to be had for the bioassay of toxins utilizing biosensors which are in keeping with the photochemical job. the information offered during this e-book serves as a foundation for the improvement of a advertisement biosensor to be used in swift pre-screening analyses of photosystem II toxins, minimising high priced and time-consuming laboratory analyses.
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Extra info for Biotechnological Applications of Photosynthetic Proteins: Biochips, Biosensors and Biodevices
Both proteins are integral membrane protein components of PSII. CP47 is encoded by the psbB gene while the/>j^C gene encodes CP43. ^"^ Hydropathy analyses indicate that each protein contains six transmembrane helices with the C- and N-terminal ends exposed at the stromal surface. Both proteins bind about 15 chlorophyll a molecules and 2 to 3 P-carotenes each, ^' with the majority or all of the pigments being located in the transmembrane regions where a number of conserved histidyl residues are CP47 and CP43 contain twelve and ten histidines respectively.
Isotopic labelling of the polypeptide subunits of the isolated photosystem II reaction-center complex of Chlamydomonas reihnhardtii suggests an aP heterodimeric structure for cytochrome b-550. J Photochem Photobiol 1999; 48:148-153. 56. Kunstner P, Guardiola A, Takahashi Y et al. A mutant strain of Chlamydomonas reinhardtii lacking the chloroplast photosystem II psbl gene grows photoautotrophically. J Biol Chem 1995; 270:9651-9654. 57. Ikeuchi M, Shukla VK, Pakrasi HB et al. Directed inactivation of the psbl gene does not affect photosystem II in the cyanobacterium Synechocystis sp.
Lindberg K, Wydrzynski T, Vanngard T et al. Slow release of chloride from ^ Cl-labeled photosystem II membranes FEBS Lett 1990; 264:153-155. 128. Lindberg K, Andr^asson L-E. A One-Site, Two-State Model for the Binding of Anions in Photosystem II. Biochemistry 1996; 35:14259-14267. 129. Wincencjusz H, van Gorkom HJ, Yocum CF. ^2 transitions. Biochemistry 1997; 36:3663-3670. 130. Wincencjusz H, Yocum CF, van Gorkom HJ. Activating anions that replace CI' in the 02-evolving complex of photosystem II slow the kinetics of the terminal step in water oxidation and destabilize the S2 and S3 states Biochemistry 1999; 38:3719-3725.