By John G. Taylor
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Extra info for Bioengineering for Pollution Prevention
Enzymatic kinetic models. Detailed kinetic models, involving descriptions of enzyme characteristics, fall into three broad categories. The most detailed and comprehensive Biotechnological Platforms 25 are referred to as mechanistic or structured models. In such models, rate expressions are proposed that attempt to describe the mechanistic role of each enzyme in the metabolic pathway. A fundamental structured model also includes information about characteristic microbial cell dimensions and effects of rate-limiting mass transfer across cell walls.
The tac promoter: a functional hybrid derived from the trp and lac promoters, Proc Natl Acad Sci 80:21-25. , and J. Hoeffler, Eds. (1999). Gene Expression Systems: using Nature for the Art of Expression. Academic Press. , Ed. (2004). Protein Expression Technologies: Current Status and Future Trends. Horizon Bioscience. Manoharan, M. (2004). RNA interference and chemically modified small interfering RNAs, Curr Opin Chem Biol 8:570-579. , and M. Provenzano (2004). RNA interference: learning gene knockdown from cell physiology, J Transl Med 2:39.
Fortunately, new quasi-linear algebra methods have been developed to calculate fluxes from large data sets and, importantly, to estimate sensitivities to measurement errors . A number of systematic descriptions of metabolic pathways for E. coli have been developed using these methods [11-13]. 2. Enzymatic kinetic models. Detailed kinetic models, involving descriptions of enzyme characteristics, fall into three broad categories. The most detailed and comprehensive Biotechnological Platforms 25 are referred to as mechanistic or structured models.