Animal nutrition with transgenic plants by Gerhard Flachowsky

By Gerhard Flachowsky

This ebook comprises 15 chapters that debate using transgenic vegetation as animal feeds. themes contain the restrictions of transgenic crops for animal meals; basics of plant biotechnology; dietary, security and compositional evaluate of feeds from transgenic crops; feeding reports utilizing transgenic vegetation; the destiny of transgenic DNA and expressed proteins; the influence of feeds from transgenic vegetation at the composition and caliber of animal items; feed ingredients from transgenic microorganisms; socioeconomics and public reputation of transgenic crops. on hand In Print

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Currently, there are two major techniques for transferring foreign DNA into an organism: right border (RB), consisting of a 25 bp repeat sequence, and only the DNA segment in between the two border sequences is transferred to the plant cell. When A. tumefaciens attaches to a plant cell, it transfers this T-DNA from the bacterium through a cytoplasmatic bridge to the plant cell.  The first method is based on the indirect physical transfer of foreign genes into target plant cells. The method used mostly is particle bombardment (biolistics).

2 GMO Technology: What, How and Its Importance to Plant Breeding This section describes the different steps of developing a transgenic crop. The description will be general and rather theoretical. Depending on the crop and the gene of interest to be introduced, the procedure is slightly different. However, in general terms, it can be stated that the whole process of developing a commercial GMO variety consists of the following steps: isolation and functional analysis of genes encoding a particular trait; the assembly of a functional gene construct; transfer and integration of the gene construct into the genome; identification of elite events and breeding with the elite events; and preparation of the authorization dossier, needed for commercialization.

2. The coding sequence of the gene of interest. Often, this coding sequence is adapted in order to achieve a better expression in the transgenic plant. For example, in the case of certain bacterial genes, the adenine thymine (AT) content of the coding sequence is much higher than in plants and this results in aberrant premature transcription termination. This has been the case for the gene encoding the Bacillus thuringiensis-derived insecticidal protein (BT). By substituting A–T nucleotide pairs with G–C nucleotide pairs in the coding sequence without significantly changing the amino acid sequence, correct transcription could be obtained.

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