By Tong-Cun Zhang, Motowo Nakajima
At the ICAB 2014, researchers from all over the world will assemble to debate the most recent clinical examine, findings and applied sciences bearing on Microbial Genetics and Breeding, Optimization and regulate of organic tactics, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will supply a platform for tutorial alternate at the program of biotechnology among family and overseas universities, study institutes, company specialists and students. The members will specialize in the foreign improvement and destiny tendencies. the development will lay a superior starting place for addressing key technical demanding situations in a number of parts of utilized biotechnology, offering possibilities to advertise the improvement and enlargement of the biotechnology industry.
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2; B: acetonitrile/MeOH (1:1 by vol). Ultraviolet detector was used to determine the product at 340 nm. LC-MASS was used to identiﬁcation molecular weight of the production. 7 GenBank Accession Numbers at NCBI The obtained sequences of LeuDH and FDH were performed at the website of NCBI. The GenBank accession number of leudh is KM454878, and the number of fdh is KM454879. 26 J. Bai et al. 1 Construction of LeuDH and FDH Expression Strains Strain containing pLeuDH used for expressing LeuDH was constructed.
70 × 104/L affected by light, temperature, nutrients, and other environmental factors. 09 mg/L at Center West. The signiﬁcant seasonal fluctuation meant that M. novacekii favored high temperature, which is the same habit with universal Microcystis algae. 30 clones of M. novacekii were collected and ampliﬁed the 16S rDNA fragments. Results proved that the molecular identiﬁcation of Microcystis, which is based on the conservative fragment of 16S rDNA, was consistent with the traditional morphology judgement, we conﬁrmed that environmental factors have a huge impact on the distribution of Microcystis and the molecular biology techniques based on 16S rDNA sequencing were reliable for Microcystis identiﬁcation.
Hence the research in Microcystis distribution spatially and temporally has been conducted. In this study, we focus on Microcystis aeruginosa, which is one of the most contributing causes of the bloom. Samples are collected from seven sites in July and October 2012 and molecular techniques conducted to identify the selected clone from the samples by 16S rDNA sequencing. According to the results, M. 04 × 104/L. 13 × 104/L respectively). By analyzing the sequencing results and comparing with the morphological characters, the accuracy of molecular identiﬁcation based on the 16S rDNA region is conﬁrmed.